8/9/2023 0 Comments Serial dilution![]() ![]() A number of spread plates is needed because the exact number of live bacteria in the sample is usually unknown. 10-2 to 10-8 ) is normally performed on the sample culture and spread plates created from the dilutions. If the concentration of the original stock solution was 100 µg/µL, the concentration in Tube 4 would beġ00 µg/µL × #1/160000# = 6. Figure 1: Serial dilution series and plating. The dilution factor after four dilutions is The ASSIST is a highly capable, easy-to-use, affordable automated pipetting platform designed to perform serial dilutions in microplates. Repeat the process until you have four tubes. Automating a serial dilution on a 96 well plate with ASSIST. Then transfer 0.2 mL from Tube 2 to 3.8 mL of diluent in Tube 3 and mix. Explanation: A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. You would transfer 0.2 mL from Tube 1 to 3.8 mL of diluent in Tube 2 and mix. Introduction Estimation of colony forming units (cfu) through serial dilution plating on a nutrient medium forms the most widely accepted method for monitoring cultivable bacteria and yeasts in different spheres of microbiology 14, 27. ![]() So you multiply each successive dilution by the dilution factor. Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. If you did the above dilution four times, what would be the final dilution factor? What is the dilution factor if you add 0.2 mL of a stock solution to 3.8 mL of diluent? ![]() The dilution factor or the dilution is the initial volume divided by the final volume.įor example, if you add a 1 mL sample to 9 mL of diluent to get 10 mL of solution, In serial dilutions, you multiply the dilution factors for each step. A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. ![]()
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